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epidermal growth factor  (R&D Systems)


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    Structured Review

    R&D Systems epidermal growth factor
    Epidermal Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+epidermal+growth+factor/Recombinant+Human+EGF+Protein%2C+CF/pmc13132546-182-24-27
    Average 97 stars, based on 1285 article reviews
    epidermal growth factor - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: A microbiota-derived bile acid modulates biofilm formation by the probiotic strain Escherichia coli Nissle 1917
    Article Snippet: After establishment of the organoid line, organoids were routinely grown in 3D culture according to established protocols in Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (R&D Systems, catalog no. 3533-005-02) or Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning, catalog no. 356231), dispensed as 14–15 μL droplets for a total of 100 μL/well in 12-well non-tissue-culture-treated plates or 200 μL/well in 6-well non-tissue-culture-treated plates. .. The droplets were overlaid with expansion medium (1 mL/well for 12-well plates, 2 mL/well for 6-well plates) consisting of Advanced DMEM/F12 (Gibco, catalog no. 12634028), 10 mM HEPES buffer (pH 7.0; Gibco, cat. no. 15630-056), 1× GlutaMAX supplement (Gibco, cat. no. 35050-038), 1× B-27 supplement (Gibco, catalog no. 17504044), 1.25 mM N-acetylcysteine (Sigma-Aldrich, catalog no. A9165), 10 mM nicotinamide (Sigma-Aldrich, catalog no. N0636), 100 ng/mL recombinant human Noggin (R&D Systems, catalog no. 6057-NG), 50 ng/mL recombinant human epidermal growth factor (R&D Systems, catalog no. 236-EG), 5 nM gastrin (Tocris, catalog no. 3006), 0.5 μM ALK5 inhibitor A83-01 (Tocris, catalog no. 2939), 10 μM p38 inhibitor SB202190 (Sigma-Aldrich, catalog no. S7076), 250 ng/mL recombinant human R-Spondin 3 (RSPO3; produced in-house or obtained from R&D Systems, catalog no. 3500-RS), 1 μM prostaglandin E2 (PGE2; Tocris, catalog no. 2296), 0.5 nM Wnt surrogate-Fc fusion protein (Gibco, catalog no. PHG0402), and 5% heat-inactivated fetal bovine serum (FBS, Gibco A5209501). ..

    Article Title: Macula Densa Alleviates Shiga Toxin-Induced Acute Kidney Injury via CCN1-Mediated Renal Tubular Repair.
    Article Snippet: .. Cells were resuspended in Kidney Culture Medium consisting of DMEM/F-12 (Gibco, Grand Island, NY, USA) supplemented with insulin (5 μg/mL), transferrin (2.75 μg/mL), selenium (3.35 ng/mL) (ITS; Invitrogen, Carlsbad, CA, USA), hydrocortisone (40 ng/mL; Sigma-Aldrich), recombinant human epidermal growth factor (10 ng/mL; R&D Systems, Minneapolis, MN, USA), and 1% antibiotic-antimycotic solution (10,000 U/mL penicillin, 100 μg/mL streptomycin, 0.25 μg/mL amphotericin B; Sigma-Aldrich). ..

    Article Title: Sodium–Glucose Cotransporter 2 and Glucose Levels Affect Clear Cell Renal Cell Carcinoma Progression
    Article Snippet: Briefly, cells (1 × 10 3 /well) were seeded in six-well tissue culture plates and incubated until colonies were detected in the control well. .. Cells cultured in serum-free media or treated with 10 ng/mL recombinant human epidermal growth factor (R&D Systems, Minneapolis, MN, USA) served as negative and positive controls, respectively. .. After fixing the cells/colonies with methanol and staining with 0.1% crystal violet, each well was photographed to facilitate colony quantification using ImageJ software version 1.54 (National Institutes of Health, Bethesda, MD, USA).

    Article Title: The PCNA inhibitor AOH1996 suppresses cancer stemness and enhances anti-PD1 immunotherapy in squamous cell carcinoma.
    Article Snippet: Serum-free DMEM/F12 (Thermo Fisher Scientific, Shanghai, China; Cat# 8122659) supplemented with 1% N2 (Thermo Fisher Scientific; Cat# 17502048) and 1% B27 supplement (Thermo Fisher Scientific; Cat# 17504044) was used to culture the cells. .. A total of 20 ng/mL recombinant human epidermal growth factor (EGF; R&D Systems, Minneapolis, MN, USA; Cat# 236-EG-01 M) and 10 ng/mL human basic fibroblast growth factor (FGF; R&D Systems; Cat# 233-FB/CF) [33] were added to the medium. ..

    Article Title: Sodium-Glucose Cotransporter 2 and Glucose Levels Affect Clear Cell Renal Cell Carcinoma Progression.
    Article Snippet: .. Cells cultured in serum-free media or treated with 10 ng/mL recombinant human epidermal growth factor (R&D Systems, Minneapolis, MN, USA) served as negative and positive controls, respectively. .. After fixing the cells/colonies with methanol and staining with 0.1% crystal violet, each well was photographed to facilitate colony quantification using ImageJ software version 1.54 (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Macula Densa Alleviates Shiga Toxin-Induced Acute Kidney Injury via CCN1-Mediated Renal Tubular Repair
    Article Snippet: .. Cells were resuspended in Kidney Culture Medium consisting of DMEM/F-12 (Gibco, Grand Island, NY, USA) supplemented with insulin (5 μg/mL), transferrin (2.75 μg/mL), selenium (3.35 ng/mL) (ITS; Invitrogen, Carlsbad, CA, USA), hydrocortisone (40 ng/mL; Sigma-Aldrich), recombinant human epidermal growth factor (10 ng/mL; R&D Systems, Minneapolis, MN, USA), and 1% antibiotic-antimycotic solution (10,000 U/mL penicillin, 100 μg/mL streptomycin, 0.25 μg/mL amphotericin B; Sigma-Aldrich). ..

    Article Title: A microbiota-derived bile acid modulates biofilm formation by the probiotic strain Escherichia coli Nissle 1917.
    Article Snippet: After establishment of the organoid line, organoids were routinely grown in 3D culture according to established protocols106 in Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (R&D Systems, catalog no. 3533-005-02) or Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning, catalog no. 356231), dispensed as 14–15 μL droplets for a total of 100 μL/well in 12-well non-tissueculture-treated plates or 200 μL/well in 6-well non-tissue-culture-treated plates. .. The droplets were overlaid with expansion medium (1mL/well for 12-well plates, 2 mL/well for 6-well plates) consisting of AdvancedDMEM/ F12 (Gibco, catalog no. 12634028), 10mM HEPES buffer (pH 7.0; Gibco, cat. no. 15630-056), 1×GlutaMAX supplement (Gibco, cat. no. 35050-038), 1× B-27 supplement (Gibco, catalog no. 17504044), 1.25mM N-acetylcysteine (Sigma-Aldrich, catalog no. A9165), 10mMnicotinamide (Sigma-Aldrich, catalog no. N0636), 100 ng/mL recombinant human Noggin (R&D Systems, catalog no. 6057-NG), 50 ng/mL recombinant human epidermal growth factor (R&D Systems, catalog no. 236-EG), 5 nM gastrin (Tocris, catalog no. 3006), 0.5 μM ALK5 inhibitor A83-01 (Tocris, catalog no. 2939), 10 μM p38 inhibitor SB202190 (Sigma-Aldrich, catalog npj Biofilms and Microbiomes | (2025) 11:225 15 no. S7076), 250 ng/mL recombinant human R-Spondin 3 (RSPO3; produced in-house or obtained fromR&DSystems, catalog no. 3500-RS), 1 μM prostaglandin E2 (PGE2; Tocris, catalog no. 2296), 0.5 nMWnt surrogateFc fusion protein (Gibco, catalog no. PHG0402), and 5% heat-inactivated fetal bovine serum (FBS, Gibco A5209501). ..

    Article Title: The PCNA inhibitor AOH1996 suppresses cancer stemness and enhances anti-PD1 immunotherapy in squamous cell carcinoma
    Article Snippet: Serum-free DMEM/F12 (Thermo Fisher Scientific, Shanghai, China; Cat# 8122659) supplemented with 1% N2 (Thermo Fisher Scientific; Cat# 17502048) and 1% B27 supplement (Thermo Fisher Scientific; Cat# 17504044) was used to culture the cells. .. A total of 20 ng/mL recombinant human epidermal growth factor (EGF; R&D Systems, Minneapolis, MN, USA; Cat# 236-EG-01 M) and 10 ng/mL human basic fibroblast growth factor (FGF; R&D Systems; Cat# 233-FB/CF) [ ] were added to the medium. ..

    Produced:

    Article Title: A microbiota-derived bile acid modulates biofilm formation by the probiotic strain Escherichia coli Nissle 1917
    Article Snippet: After establishment of the organoid line, organoids were routinely grown in 3D culture according to established protocols in Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (R&D Systems, catalog no. 3533-005-02) or Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning, catalog no. 356231), dispensed as 14–15 μL droplets for a total of 100 μL/well in 12-well non-tissue-culture-treated plates or 200 μL/well in 6-well non-tissue-culture-treated plates. .. The droplets were overlaid with expansion medium (1 mL/well for 12-well plates, 2 mL/well for 6-well plates) consisting of Advanced DMEM/F12 (Gibco, catalog no. 12634028), 10 mM HEPES buffer (pH 7.0; Gibco, cat. no. 15630-056), 1× GlutaMAX supplement (Gibco, cat. no. 35050-038), 1× B-27 supplement (Gibco, catalog no. 17504044), 1.25 mM N-acetylcysteine (Sigma-Aldrich, catalog no. A9165), 10 mM nicotinamide (Sigma-Aldrich, catalog no. N0636), 100 ng/mL recombinant human Noggin (R&D Systems, catalog no. 6057-NG), 50 ng/mL recombinant human epidermal growth factor (R&D Systems, catalog no. 236-EG), 5 nM gastrin (Tocris, catalog no. 3006), 0.5 μM ALK5 inhibitor A83-01 (Tocris, catalog no. 2939), 10 μM p38 inhibitor SB202190 (Sigma-Aldrich, catalog no. S7076), 250 ng/mL recombinant human R-Spondin 3 (RSPO3; produced in-house or obtained from R&D Systems, catalog no. 3500-RS), 1 μM prostaglandin E2 (PGE2; Tocris, catalog no. 2296), 0.5 nM Wnt surrogate-Fc fusion protein (Gibco, catalog no. PHG0402), and 5% heat-inactivated fetal bovine serum (FBS, Gibco A5209501). ..

    Article Title: A microbiota-derived bile acid modulates biofilm formation by the probiotic strain Escherichia coli Nissle 1917.
    Article Snippet: After establishment of the organoid line, organoids were routinely grown in 3D culture according to established protocols106 in Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (R&D Systems, catalog no. 3533-005-02) or Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning, catalog no. 356231), dispensed as 14–15 μL droplets for a total of 100 μL/well in 12-well non-tissueculture-treated plates or 200 μL/well in 6-well non-tissue-culture-treated plates. .. The droplets were overlaid with expansion medium (1mL/well for 12-well plates, 2 mL/well for 6-well plates) consisting of AdvancedDMEM/ F12 (Gibco, catalog no. 12634028), 10mM HEPES buffer (pH 7.0; Gibco, cat. no. 15630-056), 1×GlutaMAX supplement (Gibco, cat. no. 35050-038), 1× B-27 supplement (Gibco, catalog no. 17504044), 1.25mM N-acetylcysteine (Sigma-Aldrich, catalog no. A9165), 10mMnicotinamide (Sigma-Aldrich, catalog no. N0636), 100 ng/mL recombinant human Noggin (R&D Systems, catalog no. 6057-NG), 50 ng/mL recombinant human epidermal growth factor (R&D Systems, catalog no. 236-EG), 5 nM gastrin (Tocris, catalog no. 3006), 0.5 μM ALK5 inhibitor A83-01 (Tocris, catalog no. 2939), 10 μM p38 inhibitor SB202190 (Sigma-Aldrich, catalog npj Biofilms and Microbiomes | (2025) 11:225 15 no. S7076), 250 ng/mL recombinant human R-Spondin 3 (RSPO3; produced in-house or obtained fromR&DSystems, catalog no. 3500-RS), 1 μM prostaglandin E2 (PGE2; Tocris, catalog no. 2296), 0.5 nMWnt surrogateFc fusion protein (Gibco, catalog no. PHG0402), and 5% heat-inactivated fetal bovine serum (FBS, Gibco A5209501). ..

    Cell Culture:

    Article Title: Sodium–Glucose Cotransporter 2 and Glucose Levels Affect Clear Cell Renal Cell Carcinoma Progression
    Article Snippet: Briefly, cells (1 × 10 3 /well) were seeded in six-well tissue culture plates and incubated until colonies were detected in the control well. .. Cells cultured in serum-free media or treated with 10 ng/mL recombinant human epidermal growth factor (R&D Systems, Minneapolis, MN, USA) served as negative and positive controls, respectively. .. After fixing the cells/colonies with methanol and staining with 0.1% crystal violet, each well was photographed to facilitate colony quantification using ImageJ software version 1.54 (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Sodium-Glucose Cotransporter 2 and Glucose Levels Affect Clear Cell Renal Cell Carcinoma Progression.
    Article Snippet: .. Cells cultured in serum-free media or treated with 10 ng/mL recombinant human epidermal growth factor (R&D Systems, Minneapolis, MN, USA) served as negative and positive controls, respectively. .. After fixing the cells/colonies with methanol and staining with 0.1% crystal violet, each well was photographed to facilitate colony quantification using ImageJ software version 1.54 (National Institutes of Health, Bethesda, MD, USA).



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